Group of marine microbial

autotrophic, heterotrophic pico-, nano- and microplankton
Papanin Institute for Biology of Inland Waters RAS, Borok, Russia
Alexander Kopylov
Elena Zabotkina
P.P. Shirshov Institute of Oceanology RAS, Moscow, Russia
Andrey Sazhin
Nadezda Romanova

Objects
Virioplankton (abundance of viral particles, viruses attached to bacterial cells, capsid size, frequency of infected bacterial cells, burst size, viral-mediated bacterial mortality)
Bacterioplankton (abundance, biomass, bacterial production and contribution of attached cells to the total abundance)
Autotrophic and heterotrophic flagellates and community structure of phytoplankton
Ciliates and other Protists, biomass and species composition

methods
The number and size of the bacterial cells (stained with DAPI or SYBR Green I) are estimated using epifluorescence microscopy and flow cytometry.
Phytoplankton and heterotrophic protists (stained with primulin) are detected by epifluorescence microscopy.
Virus-like particles are counted by epifluorescence microscopy after staining with SYBR Green I. Attached viral particles, frequency of visibly infected cells, burst size and capsid size are estimated using transmission electron microscopy.