Phylgenetic tree

Слайд 2

>70 % identity

>85% identity

~60% identity

>70 % identity >85% identity ~60% identity

Слайд 3

1. Expression profile- roots (R), leaves (L), Se –treated with NaCl,

1. Expression profile- roots (R), leaves (L), Se –treated with NaCl, 4oC,
4oC, H2O stress
2. Available knock-out mutants–Y/homozygous/intron
3. Over-expression – Y
4. Promoter-GUS -Y
*putative signal peptide
mlsaflghasaiaiaspvvsivgirrqtygemkhvgvvekqr

MT-1 ( At5g37990)

Слайд 4

MT-2 ( At5g37970)

1. Expression profile – roots (R), leaves (L), Se –treated

MT-2 ( At5g37970) 1. Expression profile – roots (R), leaves (L), Se
with 4oC, H2O stress
2. Knock-out mutants-NO
3. Over-expression - YES
4. Promoter-GUS - YES
*putative signal peptide
Mlsafwghasaiaiaspvvsivgfqgdrttirrptygemkfvdvverpr – no predicted cleveage signal

Слайд 5

MT-3 ( At5g38780)

1. Expression profile- R, Leaves, Se, SeNaCl, Se4oC, Water treated

MT-3 ( At5g38780) 1. Expression profile- R, Leaves, Se, SeNaCl, Se4oC, Water
leaves
2. Available knock-out mutants-Y-homozygous/exon
3. Over-expression - Y
4. Promoter-GUS – Y
*Microarray: detected in senescent leaves /SA, JA mutants/

Слайд 6

MT-4 ( At5g38100)

1. Expression profile-St, L, F, R, Se, Se-NaCl, Se-4oC
2.

MT-4 ( At5g38100) 1. Expression profile-St, L, F, R, Se, Se-NaCl, Se-4oC
Available knock-out mutants-?-one putative, 5`UTR
3. Over-expression - Y
4. Promoter-GUS - Y

Слайд 7

MT-9 ( At5g68040)

1. Expression profile - R
2. Available knock-out mutants-Y/homozygous/exon
3. Over-expression -

MT-9 ( At5g68040) 1. Expression profile - R 2. Available knock-out mutants-Y/homozygous/exon
Y
4. Promoter-GUS - Y

Слайд 8

MT-12 ( At5g56300)

1. Expression profile-developing seeds, F, R, L, Se, Se-NaCl, Se-4oC
2.

MT-12 ( At5g56300) 1. Expression profile-developing seeds, F, R, L, Se, Se-NaCl,
Available knock-out mutants -Y/heterozygous
3. Over-expression - Y
4. Promoter-GUS - Y

Слайд 9

MT-13 ( At5g26420)

1. Expression profile-exclusively in developing seeds
2. Available knock-out mutants-? two

MT-13 ( At5g26420) 1. Expression profile-exclusively in developing seeds 2. Available knock-out
putative mutants
3. Over-expression - Y
4. Promoter-GUS -Y

Слайд 10

Back to the first

MT-12
MT-13

MT-1,2,3,4,9,12
MT-3
MT-4
MT-12

Se

Roots

Developing siliques

Flower

LEAVES

MT-4
MT-12
MT-4
MT-12
MT-9?
MT-3?

MT-1, MT-2

Back to the first MT-12 MT-13 MT-1,2,3,4,9,12 MT-3 MT-4 MT-12 Se Roots