Rutilus Rutilus Ukraine 2022
2 Materials & Methods: aim, object, sampling large fast-growing forms group I small slow-growing group II Kaniv reservoir Forms of roach (Rutilus rutilus) The aim of the study was to investigate the genetic structure of two forms of roach (large fast-growing forms – group I and small slow-growing – group II) of the Kaniv reservoir using microsatellite loci (Rru: 3, 4 and 7; Са: 3, 5 and 12). ♀ ♀ ♂ ♂ Notes: * - Temperature was optimized F - forward primer's sequence; R - reverse primer's sequence; D - denaturation; AT - annealing temperature; E - extension. C - Cycles Separation in 8% PAGE Gel analysіs in Totallab v.2.01 Statistical Analysis in Genalex 6.5 (Peakall & Smouse, 2006, 2012). PIC (The polymorphic information content ) was calculated using the formulas generally accepted for codominant markers (Nagy et al., 2012). Materials & Methods: Microsatellite Analysis 3 1. Barinova,A., Yadrenkina,E., Nakajima,M. and Taniguchi,N. (2004). Identification and characterization of microsatellite DNA markers developed in ide Leuciscus idus and Siberian roach Rutilus rutilus. Mol. Ecol. Notes 4, 86-88 2. Dimsoski P. , Toth G. P. , Bagley M. J. (2000) Microsatellite characterization in central stoneroller Campostoma anomalum (Pisces: Cyprinidae). Blackwell Science Ltd, Molecular Ecology, 9, 2187–2189 3. Сrooijmans R., Bierbooms V., Komen J. et al. Miсrosatellite markers in common carp (Cyprinus carpio L.). Animal Genetics. 1997. V. 28. P. 129 – 134. 4.Gheyas A. A., Cairney M., Gilmour A. E., Sattar M. A., Das T. K., Mcandrew B. J., Penman D. J. and Taggart J. B. (2006) Characterization of microsatellite loci in silver carp (Hypophthalmichthys molitrix), and cross-amplification in other cyprinid species. Molecular Ecology Notes 6, 656–659. doi: 10.1111/j.1471-8286.2006.01288.x © 2006
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