Rutilus Rutilus Ukraine 2022

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Materials & Methods: aim, object, sampling
large
fast-growing forms
group I
small
slow-growing
group II
Kaniv reservoir
Forms of roach (Rutilus rutilus)
The aim of the study was to investigate the genetic structure of two forms of roach (large fast-growing forms – group I and small slow-growing – group II) of the Kaniv reservoir using microsatellite loci (Rru: 3, 4 and 7; Са: 3, 5 and 12).
♀
♀
♂
♂

Notes:
* - Temperature was optimized
F - forward primer's sequence;
R - reverse primer's sequence;
D - denaturation;
AT - annealing temperature;
E - extension.
C - Cycles
Separation in 8% PAGE
Gel analysіs in Totallab v.2.01
Statistical Analysis in Genalex 6.5 (Peakall & Smouse, 2006, 2012).
PIC (The polymorphic information content ) was calculated using the formulas generally accepted for codominant markers (Nagy et al., 2012).
Materials & Methods: Microsatellite Analysis
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1. Barinova,A., Yadrenkina,E., Nakajima,M. and Taniguchi,N. (2004). Identification and characterization of microsatellite DNA markers developed in ide Leuciscus idus and Siberian roach Rutilus rutilus. Mol. Ecol. Notes 4, 86-88
2. Dimsoski P. , Toth G. P. , Bagley M. J. (2000) Microsatellite characterization in central stoneroller Campostoma anomalum (Pisces: Cyprinidae). Blackwell Science Ltd, Molecular Ecology, 9, 2187–2189
3. Сrooijmans R., Bierbooms V., Komen J. et al. Miсrosatellite markers in common carp (Cyprinus carpio L.). Animal Genetics. 1997. V. 28. P. 129 – 134.
4.Gheyas A. A., Cairney M., Gilmour A. E., Sattar M. A., Das T. K., Mcandrew B. J., Penman D. J. and Taggart J. B. (2006) Characterization of microsatellite loci in silver carp (Hypophthalmichthys molitrix), and cross-amplification in other cyprinid species. Molecular Ecology Notes 6, 656–659. doi: 10.1111/j.1471-8286.2006.01288.x © 2006

Results: evaluation of the effectiveness of markers
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Results: differences between groups
Mean 10.83 ± 2.40
Mean 7.92 ± 1.92
Mean 2.10 ± 0.20
Mean 0.87 ± 0.03
Notes:

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Results: differences in Allele Frequency between groups
5
group I
group II
І
ІI
І
ІI
І+ ІI
І+ ІI
І+ ІI
Notes:

Conclusion
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The obtained results allowed making assumptions about the different genetic origin of the studied roach groups. Confirmation of this hypothesis requires additional comparison of the genetic structure of fast-growing roach and R. heckelii inhabiting the lower Dnieper.
The studied loci were found to be polymorphic (100%) (average Ne = 7.9±1.9).
The average polymorphic information content increased in the following sequence
Ca 3 (0.657) < Rru 7 (0.685) < Rru 4 (0.723) < Ca 12 (0.797) < Rru 3 (0.803) < Ca 5 (0.883).
Since the PIC value was above 0.5, it was concluded that all SSR primers used were characterized by high polymorphism.
Key Features of studied groups
General:
There are no significant differences in the value of Shannon index I in the groups (fast-growing 1.762 ± 0.198 and slow-growing 1.717 ± 0.123)
For Ca5 locus - allelic variant of 287 bp occurred in both groups (0.050 in group I and 0.222 in group II).
For Rru7 locus - 82 bp, which occurred in both groups with a high frequency: 40% and 30%, respectively.
For Rru3 locus, the presence of a specific allele of 187 bp was found, which was typical for both groups and occurred with a frequency of 22% in group I and 30% in group II.
For Rru4 - variant of 140 bp occurring with a high frequency in both groups (30% and 45%, respectively)
Differences:
For Ca3 locus:
- in group I allelic variants of 224 bp. (50%) and 237 bp (25%) were private alleles
- іn group II - 275 bp and 297 bp variants were the most frequent (25% each), which were specific for this group.