Recombinant antibodies for diagnosis and therapy

Schematic presentation of whole IgG and different fragments

Anti –erbB2 scFv
Affinity from 10-7 to 10-9
Activity in cellular test not incriesed
Binding activity from 2 to 400 nM
Diabody and tumor targeting better for 2 nM
Anti mesothelin SS antibody (scFv) 0,8 nM 10 fold active that 11 nM,
But another mutant with 0,2 nM affinity have low activity that 0,8 nM
Binding Site Barrier
Hypothesis