Culture

Identification of microorganisms
Microscopical examination.
Culture.
Biochemical examination.

⮚In lab, bacteria must be cultured to facilitate identification & examination of growth & metabolism.
⮚Bacteria are inoculated or introduced to various forms of culture media to keep them alive.
⮚Inoculation must be under aseptic conditions to exclude contamination & other unwanted microbes.
Types of culture media:
⮚Classification is according to:
Physical state.
Chemical composition.
Functional type.
Culture

Culture
Culture media

Types of culture media:
⮚Classification is according to:
Physical state.
liquid media.
Semisolid media.
Solid media.
Chemical composition.
Syntheic media.
Non synthetic media.
Functional type.
Basic media.
Enriched media.
Selective media.
Differfntial media.
Culture

According to physical state:
Culture

According to chemical composition:
Culture

According to functional type:
Culture

Culture
Isolation
of bacteria

Inoculation & isolation of bacteria on nutrient agar:
Culture
Petri dish
B.S
Slant

Cultivation is near the flame
A
B
C
D
B.s
With flammed loop
Flam the loop
Flam the loop

Then incubate at 37°c for 24 hrs in incubator
Culture

After incubation :
Culture

Colony Description:
Culture

Colony Description:
Culture

Colony Description:
Culture
Staphylococcus aureus on N.A
Basillus subtilis on N.A

Colony Description:
Culture