Annual scientific report of pharmacognosy department. Pharmacognostic analysis of the raw materials

GERMANY
FRANCE
ITALY
2 mld $ 1 mld $
500 mln $

Alam Sher Antimicrobial activity of natural products from medicinal plants // Gomal Journal of Medical Sciences.-.2009.-.Vol.7.-.N1.-.P.72-78.
Atrea I., Papavergou A., Amvrosiadis I., Savvaidis I.N. Combined effect of vacuum-packging and oregano essential oil on the shelf-life of Mediterranean octopus (Octopus vulgaris) from the Aegean Sea stored at 40C // Food Microbiology 26.-Greece.-2009.-P.166-172.
Benavides S., Villalobos-Carvajal R., Reyes J.E. Physical, mechanical and antibacterial properties of alginate film: Effect of the crosslinking degree and oregano essential oil concentration // Journal of Food Engineering 110.-Chillon.-2012.-P.232-239.
Bermstein N.,Chaimovitch D., Dudai N. Effect of Irrigation with Secondary Treated Effluent on Essential oil, Antioxidant Activity, and Phanolic compounds in Oregano and Rosemary // Agronomy Journal, Crop Science Society of America.-Vol.101.-N1.-U.S.E.-2007.-P.1-10.
Bhargava K., Venskutonis P, Dewettinck K, Verhe R (2015) Application of an oregano oil nanoemulsion to the control of foodborne bacteria on fresh lettuce. Food Microbiology, 47:69 -73
Bisht D., Chanotiya Ch. S., Rana M., Semwal M. Variability in essential oil and bioactive chiral monoterpenoid compositions of Indian oregano (Origanumvulgare L.) populations from northwestern Himalaya and their chemotaxonomy // Industrial Crops and Products.-vol. 30.-2009.-P. 422-426.
Botsoglou N.A.,Christaki E., Fletouris D.J., Florou-Paneri P., Spais A.B. The effect of dietary oregano essential oil lipid oxidation in raw and cooked chiken during refrigerated storage // Meat Science 62.-Greece.-2002.-P. 259-265.
Busatta C., Mossi A. J., Rodrigues M.R. A., Cansian R.L., Oliveira J.V. Evaluation of Origanumvulgare essential oil as antimicrobial agebt in sausage // Brazillian Journal of Microbiology.-vol.30.-No.4.-2007.
Derwich E., Benziane Z., Manar A., Boukir A., Taouil R. Phytochemical Analysis and in vitro Antibacterial Activity of the Essential Oil of Origanumvulgare from Morroco // American-European Journal of Scientific Research.-vol. 5(2).-2010.-P.120-129.
Falco E.D., Mancini E., Roscigno G., Mignola E., Taglialatela-Scafati O., Senatore F. Chemical Composition and Biological Activity of Essential Oils of Origanum vulgare L. subsp. vulgare L. under Different Growth Conditions // Journal of Molecules, Vol. 18 (12).-P. 14948-14960.-2013.
Nuro A., Dervishi A., Peçi D., Marku E. Determination of essential oil composition of Origanum vulgare populations from south of Albania // International Journal of Agriculture, Environment and Bioresearch.-vol. 2(3).-P. 346-355.-2017.
ACTUALITY

Antropogenic factor

To study unknown and perspective plant species wildly growing in Armenian and Artsakh floras, the perspectives of their cultivation, for expansion and standardization of resins resources and effective preparations creation.

Resourcologycal
Possible volume of the annual collection
Phythochemical
Biological resources
Merchandising
Biological
Ash, moisture, mineral mixtures, extractive substances
Ecological contamination
Volatile oils, flavanoids, tannins,
phenolic glycoside, essential aminoacids
Antimicrobal, antioxidant activity
Pharmacognostic analysis
Standardization of raw materials
Valeriana cardiola L.
Teucrium polium L.
Ziziphora clinopodioides Lam.
Origanum vulgare L.
Cherophyllum bulbosum L.

Ziziphora clinopodies Lam and Teucrium polium grown under hydroponic conditions (glub)

Determination of anatomical diagnostic characteristics
of raw materials (identification of species)

Determination of anatomical diagnostic characteristics of the raw materials (identification of species)

Determination of mineral content

Determination of mineral content

Mineral constituents of Rhizomta cum radicibus Valerianae cardiola L. gathered from Zovuni and Aparan regions (n=5, P<0,05)

GC-MS analysis of essential oil of roots and rhizomes of Valerian officinalis
GC-MS analysis of essential oil of roots and rhizomes of Valerian cardiola

GC-MS analisys of essential oils obtained from Rhizomta cum radicibus Valerianae officinalis L. cultivated in Zovuni region

GC-MS essential oil (wildly, hydroponic and land cultivated)

GC-MS analysis of essential oil of T. polium

վերբասկոզիդ (1), ապիգենին (3)
HPLC analysis of wildly growing
Ziziphora clinopodies Lam.

Standardization of poliumoside, teupoliumoside in the 50% alcoholic extract of T. polium-by HPLC method
Standardization of verbascoside, luteoline and apigenin in the 50% alcoholic extract of T. polium-by HPLC method
Cromatogramme of standard solusions of verbascoside (1), luteoline (2) apigenine (3)
Chromatogramme of standard solusions 50% alcoholic extract of verbascoside, luteoline and apigenine

High-performance liquid chromatography (HPLC)
Teucrium polium (wildly and hydroponic)

Determination of antioxidant activity
Determination of antiradical 50%activity of alcoholic exrtacts of T. polium growing in different conditions
Determination of antiradical activity of alcoholic exrtacts of Ziziphora growing in different conditions Z1(Berdadzor), Z2(Vokhchaberd), Z3(Hydroponic)

Determination of antimicrobial activity

UV specter of alcoholic extract in visible region
1.
բ)
UV spectr of alcoholic extract in visible region 1-4. V. cardiola (Zovuni, Aparan), 5. T. polium, 6-9. Origanum vulgare,
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Collaboration

Published articles in 2016-2017
1. Naira Shaboyan, Nelly Ghukasyan, Vahe Hovhannisyan, Armine Moghrovyan, Hasmik Galstyan, Naira Chichoyan and Karine Dumanyan. The analysis of chemical composition of several valuable medicinal and edible plants volatile fraction growing and cultivating in Armenian flora by GC-MS method. International Journal Of Current Medical And Pharmaceutical Research, Vol. 2, Issue, 4, 2016, pp.274-278.
2. Sinanyan Ara, Naira Chichoyan. Chemical Composition and Antimicrobial Activity of Wild-Harvested Achillea millefolium from the Republic of Armenia. Scripps Natural Supplements conference (San Diego, California, USA), 2016.
3. Վ. Գ. Հովհաննիսյան, Չիչոյան Ն. Բ., Գալստյան Հ. Մ., Դումանյան Կ. Հ., Շաբոյան Ն. Կ., Ղուկասյան Ն. Հ., Մողրովյան Ա. Վ. «The analysis of chemical composition of several valuable medicinal and edible plants volatile fraction growing and cultivating in Armenian flora by GC-MS method», International Journal of Current Medical and Pharmaceutical Research, Vol. 2, Issue 4, April, 2016, p. 274-278:
4. Галстян А. М., Чичоян Н. Б., Думанян К. Г., Изучение химического состава эфирного масла Дубровника беловойлочного (Teucrium polium L.) произрастающего в условиях гидропоники, почвы и в диком виде, Բժշկություն, գիտություն և կրթություն, Երևան, 2016, N 21, 160-164
5. Ulikhanyan G.R., Chichoyan N.B., Galstyan H.M., Martirosyan S.S., Ulikhanyan Gh.I., The study of the chemical composition of essential oil ziziphora clinopodioides Lam. By Gas Chromatography-Mass Spectrometry Method of the Armenian flora, VІ Международная научно-практическая конференция, «Актуальные проблемы науки ХХІ века», 3 часть, Москва, 2016 г,142-149.
6. Л. В. Даниелян, Н. Б. Чичоян, Г. Г. Оганесян. Усвоение лактулозы и различных растительных камедей молочнокислыми бактериями, выделенными из грудного молока. Медицинская наука Армении, т. LVII, N 3, 2017, с. 59-66.
7. Վ.Գ. Հովհաննիսյան. Զովունու եւ Ապարանի շրջաններում մշակվող կատվախոտ դեղատուի (Valeriana officinalis L.) եւ կատվախոտ սրտայինի (Valeriana cardiola L.) կոճղարմատներ՝ արմատներով հումքում վալեպոտրիատների քանակական վերլուծությունը. Հայաստանի Կենսաբանական Հանդես, ՀՀ ԳԱԱ “Գիտություն” հրատարակչություն, 2017թ., N 3, էջ 75-79.

9. H.M. Galstyan, N.B. Chichoyan, K.H. Dumanyan, G.R. Ulikhanyan. Optimization of a mineral nutrition and the effect of planting density on the productivity of Teucrium Polium (Teucrium polium L.) in an open hydroponics. Health Education Research, Australia, Melbourne, 2017, vol. 32, p. 1626-1632.
10. G.R. Ulikhanyan, N.B. Chichoyan, A.M. Galstyan, Gh.I. Ulikhanyan. Development method of Ziziphora Clinopodioides Lam. Standardization by flavonoids. Health Education Research, Australia, Melbourne, 2017, vol. 32, p. 1633-1639
11. Վ.Գ. Հովհաննիսյան, Ն.Բ. Չիչոյան, Հ.Մ. Գալստյան. The farmacognostic research of Valeriana officinalis L. and Valeriana cardiola L. rhizome and roots cultivating in different regions of Armenia. IV International Scientific Conference of Young Researchers "Biotechnology: Science and Practice" that will take place in the SPC “Armbiotechnology” NAS RA, Yerevan (Armenia) from 28 to 30 September 2017
12. Գ.Ռ. Ուլիխանյան, Ն.Բ.Չիչոյան. Сравнительная антирадикальная активность экстрактов зизифоры пахучковидной (ziziphora clinopodioides lam.) дикорастущей и выращенной в условиях гидропоники. VIII Международная конференция современные аспекты реабилитации в медицине Ереван, РА, Степанакерт, НКР 13 - 15 сентября 2017 года.
13. Հովհաննիսյան Վ.Գ., Չիչոյան Ն.Բ., Գալստյան Հ. Մ., Դումանյան Կ.Հ. Հայաստանի տարբեր շրջաններում մշակվող կատվախոտ դեղատու (Valeriana officinalis L.) եւ կատվախոտ սրտային (Valeriana cardiola L.) տեսակներից մթերված հումքերի (Rhizoma cum radicibus) ֆարմակոգնոստիկ վերլուծությունը. “Բժշկություն, գիտություն, կրթություն”, Երեւան, N23, 2017, 18-24
14. V. Hovhannisyan, N. Chichoyan, H. Galstyan “Pharmacognostic analysis of Valerianaofficinalis L. and Valerianacardiola L. rhizome and roots cultivated in Armenia”, Biotechnology: Science and Practice, September 28-30, 2017, Yerevan, Armenia, p. 54-55

Conclusions
The populations and raw material resources of several perspective medicinal species growing wildly in Armenia and Artsakh were investigated.
In wild nature the types of the species were defined after identification and regulatory numbers were given to them.
By classical and modern methods the raw materials harvested from cultivating and wildly growing perspective species were standardized according to the containing active compounds (flavonoids, tannins, phenolic glycosides).
Some medicinal species were successfully acclimatized in wild nature and introduced in hydroponic culture.