panels
show ventral views of the distal tip of the embryo, the position of the node. Anterior is to the left.
A,B: MS (mid-streak, e7.25) stage. Visceral endoderm covers distal region
of the embryo.
C,D: LS stage (e7.5). Clusters of cells with small apical surfaces and cilia begin to
appear in groups near the presumptive node (arrows, C) and the axial midline (arrowheads, C). Some endoderm cells lie over the node field (arrowhead, D). Most cilia are located in the center of node cells at this stage.
E,F: 0B stage (e7.5– e7.75). The node region is free of overlying endoderm but has not adopted a concave shape. Cilia are longer and continue to project outward.
G,H: EHF stage (e7.75). The pit of the node is concave; cilia have elongated and project posteriorly. The panel in G is a lower magnification view of the embryo shown in Figure 3A.
These images are from
embryos in the C3H/HeJ inbred strain. Scale bars 20 m in A,C,E,G, 2 m in B,D,F,H.
Lee, Anderson DEV. DYNAMICS 237:3464–3476, 2008
Lee, Anderson DEV. DYNAMICS 237:3464–3476, 2008