Применение лазеров в медицине

Перспективные современные исследования на клеточном и молекулярном уровне обязательно включают этап лазерных технологий.
Технологии световой микроскопии с применением лазеров постепенно переходят на новый уровень – исследования в объеме.

CLARITY (Clear, Lipid-exchanged, Anatomically Rigid, Imaging/immunostaining compatible, Tissue hYdrogel)
It was developed by Karl Deisseroth and his colleagues at the Stanford University School of Medicine.
Brain structures visualizing using CLARITY protocol for immunochemical studying of optically transparent mouse brain

PSD-95, DARPP-32 staining. Cortex and striatum region
DARPP32
PSD-95
Merge
Clarity
coronal slice
Striatum
Cortex
A
C
B
E
D
PSD-95. Hippocampus
Merge

Thy1-GFP M-line
Hippocampal neurons
3D reconstruction

Ontogenetic project
Step 1: transfection
Cell culture: HEK-293T
Use: ChR2
(Channelrhodopsin2)
Control: RFP (green light)
Step 2: activation opsins with light
Recording: patch-clamp
Configuration: whole-cell
Light: blue (470nm)
Change configuration under light
Blue light stimulation