Analysis of proteins

Fig. 1. The diffusion dependence of solid-phase immunoassay and methods used to reduce
its influence. (A) The effect of vortexing (shaking) microtiters wells on establishment of
equilibrium (from ref. 13). (B) Illustration of the physical effect of vortexing microtiter wells
(rotary agitation) on the distribution of the fluid phase relative to the solid phase. The fluid
phase is depicted by wavy lines. (C) Alternative methods of confining the reaction volume to
within close proximity to the solid phase bearing the immobilized reactant.